Methods of construction Transcriptome

transcriptomics techniques include dna microarrays , next-generation sequencing technologies called rna-seq. transcription can studied @ level of individual cells single-cell transcriptomics.

there 2 general methods of inferring transcriptome sequences. 1 approach maps sequence reads onto reference genome, either of organism (whose transcriptome being studied) or of closely related species. other approach, de novo transcriptome assembly, uses software infer transcripts directly short sequence reads.